fluorescence microscopy (Beyotime)
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fluorescence microscopy
Fluorescence Microscopy, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pm40471885-99-2-19
Average 99 stars, based on 32308 article reviews
Fluorescence Microscopy, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pm40471885-99-2-19
Average 99 stars, based on 32308 article reviews
fluorescence microscopy - by Bioz Stars,
2026-09
99/100 stars
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Blocking Assay:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Incubation:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: Oleic acid improves pathological changes in Aβ1–42-induced astrocytes and Alzheimer’s disease mouse models through PKA/ACACB/CPT1A Article Snippet: For immunohistochemistry, DAB staining was carried out using a DAB substrate kit (Beyotime Biotechnology, China), and color development was allowed to proceed for 1 min at room temperature before the sections were observed under a light microscope (Nikon, Japan). .. After incubation with the fluorescent secondary antibody, nuclei were counterstained with Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Fluorescence:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: The Role of SERPINE1 in Coordinating Osteogenesis and Angiogenesis of hDPSCs for Bone Regeneration. Article Snippet: Aim: The repair of criticalsized bone defects remains a significant clinical challenge due to the difficulty in achieving spatiotemporal coordination between osteogenesis and angiogenesis.. This study aimed to identify key molecular regulators bridging these processes in human dental pulp stem cells (hDPSCs) and to evaluate the therapeutic efficacy of a bioinspired delivery system comprising SERPINE1loaded hydroxyapatite/chitosan microspheres (HA/CS MS) for vascularised bone regeneration.. Methodology: Temporal transcriptomic profiling of hDPSCs during osteogenic and angiogenic differentiation was employed to screen for candidate dualregulators. Article Title: Oleic acid improves pathological changes in Aβ1–42-induced astrocytes and Alzheimer’s disease mouse models through PKA/ACACB/CPT1A Article Snippet: For immunohistochemistry, DAB staining was carried out using a DAB substrate kit (Beyotime Biotechnology, China), and color development was allowed to proceed for 1 min at room temperature before the sections were observed under a light microscope (Nikon, Japan). .. After incubation with the fluorescent secondary antibody, nuclei were counterstained with Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Microscopy:Article Title: ZFP36L1 promotes non-small cell lung cancer progression under hypoxia by modulating CXCL9:SPP1 polarity: A single-cell transcriptomic study. Article Snippet: For M0 macrophages, cells on sterile coverslips in 24- well plates were subjected to three PBS washings, 75% ethanol fixation (15 min) and .1% Triton X-100 (Beyotime) permeabilisation (15 min). .. After 1 h of 3% BSA (Beyotime) blocking, incubation with primary antibodies continued at 4◦C overnight; after PBS washings, at RT without lights, fluorophore-conjugated secondary antibodies were introduced for 1 h. After Article Title: The Role of SERPINE1 in Coordinating Osteogenesis and Angiogenesis of hDPSCs for Bone Regeneration. Article Snippet: Aim: The repair of criticalsized bone defects remains a significant clinical challenge due to the difficulty in achieving spatiotemporal coordination between osteogenesis and angiogenesis.. This study aimed to identify key molecular regulators bridging these processes in human dental pulp stem cells (hDPSCs) and to evaluate the therapeutic efficacy of a bioinspired delivery system comprising SERPINE1loaded hydroxyapatite/chitosan microspheres (HA/CS MS) for vascularised bone regeneration.. Methodology: Temporal transcriptomic profiling of hDPSCs during osteogenic and angiogenic differentiation was employed to screen for candidate dualregulators. Article Title: A novel molecule ZYZ329 targeting histone methyltransferase SMYD3 suppresses pathological angiogenesis via the driven mitochondrial ROS/HIF-1α/VEGFA axis Article Snippet: Secondary antibodies used were: Alexa Fluor 555 conjugate anti-rabbit IgG (1:1000, 4413S, Cell Signaling Technology) or Alexa Fluor 488 conjugate anti-rabbit IgG (1:1000, 4412S, Cell Signaling Technology). .. After mounting in Article Title: Oleic acid improves pathological changes in Aβ1–42-induced astrocytes and Alzheimer’s disease mouse models through PKA/ACACB/CPT1A Article Snippet: For immunohistochemistry, DAB staining was carried out using a DAB substrate kit (Beyotime Biotechnology, China), and color development was allowed to proceed for 1 min at room temperature before the sections were observed under a light microscope (Nikon, Japan). .. After incubation with the fluorescent secondary antibody, nuclei were counterstained with Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Staining:Article Title: Rabies virus matrix protein hijacks the TGF-beta activated kinase 1 binding protein 2-p38 mitogen-activated protein kinase pathway to inhibit apoptosis and promote viral replication. Article Snippet: Rabies virus (RABV) is a neurotropic virus that infects nearly all warm-blooded mammals and is almost invariably fatal once symptoms appear.. Previous studies indicate that RABV uses its structural proteins to hijack host factors or modulate host signaling pathways to promote viral replication and pathogenicity.. In this study, we observed upregulated expression of the host factor TGF β activated kinase 1 binding protein 2 (TAB2) in N2a cells following RABV infection. Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Confocal Microscopy:Article Title: Rabies virus matrix protein hijacks the TGF-beta activated kinase 1 binding protein 2-p38 mitogen-activated protein kinase pathway to inhibit apoptosis and promote viral replication. Article Snippet: Rabies virus (RABV) is a neurotropic virus that infects nearly all warm-blooded mammals and is almost invariably fatal once symptoms appear.. Previous studies indicate that RABV uses its structural proteins to hijack host factors or modulate host signaling pathways to promote viral replication and pathogenicity.. In this study, we observed upregulated expression of the host factor TGF β activated kinase 1 binding protein 2 (TAB2) in N2a cells following RABV infection. Bicinchoninic Acid Protein Assay:Article Title: Microglial serine racemase knockout alleviates Alzheimer-like neuropathology and behavioral deficit via lactylation-mediated anti-inflammation Article Snippet: .. Penicillin–streptomycin (cat#C0222), amyloid β-peptide (1–42) (cat#P9001), lipopolysaccharide (cat#ST1470), DL-dithiothreitol (cat#ST041), the enhanced BCA protein assay kit (cat#P0010), enhanced BCA protein assay kit (cat #P0010), normal goat serum (cat #C0265), and |
![Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4487/pmc08114487/pmc08114487__12915_2021_1043_Fig1_HTML.jpg)